Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc Tris HCL (1 M), pH 8.0, 500 mL
A hybridization buffer specifically formulated for use with nylon membranes. ¿ It does not contain Denhardt¿s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. ¿ Formulated for Nylon membrane ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Denatured fragmented salmon sperm DNA may be added to this hybridization buffer to reduce nonspecific background. ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Teknova DNA Suspension Buffer, pH 8.0. RNase/DNase Tested, PCR. 250mL, Sterile. (10mM Tris, 0.1mM EDTA)
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 1M Tris-HCl solution, pH 6.8 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8
Biotang Inc TRIS-HCL Buffer 1.0M, pH 6.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 6.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.0 (0.2 micron filtered), 500 ml.
Electron Microscopy Sciences Pipes Buffer Solution 0.3m, pH 5.5-6.0 500 ML
PIPES is a hydrogen-ion organic-based buffer, used in the preparation of a variety of mammalian tissues for electron microscopy. It produces excellent results in the preservation of ultrastructural details, especially when long fixation is required. This buffer does not appear to contribute extraneous anions or cations to the tissue. Aqueous PIPES solution is adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
pH: 5.5-6.0
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
BioMed Diagnostics An isotonic solution typically used for detection of the severe acute respiratory syndrome SARS-CoV-2. | Available in: 50 pack
This Saline Solution (0.85%) has been validated for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease in humans. | Product Principle: 1.) Intended to aid in the process of collection and transport of human clinical specimen. 2.) Ensures a non-replicating competent status of SARS-CoV-2. 3.) Is aseptic and does not contain bacteria or fungi growth-inhibition agents. 4.) Validated to be compatible with the identification of SARS-CoV-2 using qRT-PCR technology | Detects: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). | Available in: 50 pack
Abcam Opti-Klear™ Live Cell Imaging Buffer 1 x , 60ML
Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275938 provides an energy source and preserves fluorescent signals.
The product is subject to the following: Abcam Restricted Use Statement